Our strategy makes it possible to generate refined DNA mutations for improved safety without sacrificing efficiency of genome editing. Targeting DNA polymerase to double-strand breaks did not increase off-targets or base substitution rates around the cleavage sites, yet increased editing efficiency in primary cells. Counteracting DNA resection was one of the mechanisms perturbing deletion sizes. In addition, templated insertions (the insertion of the nucleotide 4 nt upstream of the protospacer adjacent motif) were increased relative to other insertions. Importantly, doing so also greatly decreases the generation of long deletions, including those >2 kb. Here, we show that fusing Escherichia coli DNA polymerase I or the Klenow fragment to Cas9 greatly increases the frequencies of 1-bp deletions and decreases >1-bp deletions or insertions. Methods for generating refined mutations are desirable but currently unavailable. The possibility of generating long on-target DNA deletions poses safety risks to somatic genome editing and makes the outcomes of genome editing less predictable. Most insertions or deletions generated by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) endonucleases are short (500 bp) can be observed.
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But if you add an "anti-magnetic" force, supplied by a diamagnetic material, for example, it is possible to achieve static levitation. No matter how cleverly you arrange the magnets, the magnet you are trying to levitate will always escape. Static levitation means stable suspension of an object against gravity. Putting it more precisely, he proved that it is not possible to achieve static levitation using any combination of fixed magnets and electric charges. This was proven by a scientist named Samuel Earnshaw in the appropriately named Earnshaw's theorem. As it turns out, it is not possible to levitate a magnet just using other magnets. But in ferromagnets and paramagnets the magnetic field is much stronger than the "anti-magnetic" field due to diamagnetism.ĭiamagnetic materials have the interesting characteristic that they make magnetic levitation possible. To be precise, all materials are diamagnetic, since the formation of a repulsive force in the presence of an external magnet occurs in all materials. In the case of diamagnetic materials, the atoms that make up the material respond to a magnetic field by aligning in such a way as to weakly counter, or repel, the external field. Water and graphite are examples of diamagnetic materials. Diamagnetic materials are repelled by both poles of a magnet. Liquid oxygen is an example of a paramagnetic material. Paramagnetic materials become weakly magnetic when they are near other magnets, but lose this property when away from the magnetic field. But unlike the atoms in a ferromagnetic material, the atoms in a paramagnetic material become randomly oriented when the external magnetic field is removed. When the atoms in the paramagnetic material align, their tiny magnetic fields combine to produce a magnetic field that becomes a measurable physical characteristic of the object. The atoms in paramagnetic materials will align with a magnetic field. In the case of paramagnetic materials, the atoms that make up the material are also tiny magnets, similar to the atoms in iron. Materials can also be classified as paramagnetic and diamagnetic. If the liquid is allowed to cool, the atoms are trapped in this aligned orientation as the material solidifies (you don't have to actually melt the iron for this to occur just heating it to the point that the atoms can re-align will work). If you melt iron in a magnetic field, the iron atoms in the liquid metal will tend to align with the magnetic field. You can also make iron magnets in a lab or factory. Because the atoms tend to spontaneously align with each other in ferromagnetic materials, you can find naturally occurring magnets made of ferromagnetic material, such as iron. Ferromagnetic materials can be made into permanent magnets, like the ones holding up artwork and pictures on your refrigerator. But what makes the atoms line up? In the case of iron, and other ferromagnetic materials, the atoms tend to align spontaneously. If all of the atoms are lined up in one direction, they work together to produce a large magnetic field. In the case of iron, each of the atoms that make up the material is, itself, a very tiny magnet. Why are some materials magnetic, while other materials are not? To understand why, it is helpful to think about the materials as collections of atoms. In this science project, you will focus on the last property in this list: magnetism. If you were asked to describe a material in detail, how many properties would you list? Here are a few properties that you might include: hardness, density, electrical and thermal conductivity, flammability, melting point, corrosion resistance, color, and magnetism. The ergonomic soft-grip handle with thumb rest is comfortable to hold. 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Not to be confused with the Slap Chop slicer you see on TV, the Pampered Chef's food chopper is one of my most valuable kitchen tools. Shop Pampered Chef Homes Kitchen - Kitchen Tools at up to 70 off Get the lowest price on your favorite brands at Poshmark. It was developed by Rockstar London for Microsoft Windows and PlayStation 2, Rockstar Leeds for the PlayStation Portable, and Rockstar Toronto for the Wii. Escape the nightmare that is Carcer City and maybe you’ll learn the identity of the mysterious “Director”.Action or adventure horror game manhunt explores the depths of human depravity in a vicious, sadistic tale of urban horror continues Rockstar North’s tradition of world-class gameplay with high production values and sardonic humor explore the depths of human depravity for 1 player. Manhunt 2 is a 2007 stealth game by Rockstar Games. Rockstar, the company that brought us Grand Theft Auto and Manhunt. America is full of run-down, broken rust-belt towns where nobody cares and anything goes. It's not just happening among children: in the virtual world. Plastic bags Hilarious And I cant mention Manhunt without mentioning the soundtrack. My country of course banned it because theyre pussies but of course that couldnt stop me playing it. When I heard you could suffocate people with plastic bags I knew I needed to play it. On May 24, 2003, the website has been made public. I remember when it was first announced c. Players must make strategic choices as to which weapons to use, ranging from silent and stealthy to loud and destructive.Įveryday items may be the key to your survival, as plastic bags become instruments of strangulation, and shards of glass your defensive weapons. Manhunt - Rockstar Games They just killed Cash. Nasty is a promotional website for Manhunt owned and operated by Rockstar Games. Guided only by the whispers of a man named “The Director” through his earpiece, Cash must find whatever he can to survive the night. Manhunt 1 Now is available For Android Mobile and PPSSPP Gold Emulator also Free Download Highly Compressed APK OBB ISO CSO Zip FileĪ violent action game from Rockstar, Manhunt is the story of James Earl Cash, a man previously thought dead who wakes up in a town filled with deadly gangs. In contrast, Sistek said that in the tropical climate of Florida, dew points in the low 60s during the summer are likely a cause for celebration since much of the season has dew points in the 70s there. Thus, on the few occasions they reach that level, Seattle residents surely note the very muggy conditions." "It's a rare occasion when dew points climb into the low 60s, with mid-60s nearly unheard of. "Dew points in the Seattle area typically hover in the 40s or 50s in the summer due to our proximity to the cool waters of the Pacific Ocean," said FOX Weather meteorologist Scott Sistek, a resident of the Pacific Northwest. However, these comfort and discomfort levels can vary by region because people who live in the very humid climates of the southern United States might be able to tolerate dew points in the 60s far better than those who live in the much drier climates of the northern U.S. ‘CORN SWEAT’ IN THE MIDWEST CAN CAUSE SOME OF THE MOST OPPRESSIVE HUMIDITY IN THE U.S. On rarer occasions, dew points can even get into the 80s, mainly along the Gulf Coast or in the corn-covered Upper Midwest that’s essentially unbearable to just about anyone. Weather conditions affect everyone differently, but a good generalization is that dew points in the 50s or lower are comfortable for most people, the 60s are humid and somewhat uncomfortable and the 70s are oppressive and very uncomfortable. This table illustrates how muggy the air feels at various ranges of dew points. You’re probably not going to break a sweat at 20 degrees, even with 100% relative humidity. This is why relative humidity is practically useless when trying to determine how humid the air is outside. WHAT IS THE HEAT INDEX AND WHY IS IT IMPORTANT TO YOUR HEALTH? In general, once the dew point exceeds 60 degrees, there’s a lot of water vapor in the air, which is what gives the air that muggy feel.Īt 60% relative humidity with a temperature of 80 degrees and a dew point of 65 degrees, you’re probably going to break a sweat while doing anything strenuous – even at night or on a cloudy day – because dew points in the mid-60s are indicative of a muggy air mass. That’s indicative of a bone-dry air mass even though the relative humidity says otherwise. Using the 100% relative humidity example at an air temperature of 20 degrees, that would mean the dew point is also 20 degrees. This chart illustrates the key differences between relative humidity and dew point. The service was also graced by the Dean of the Faculty of Journalism, Media and Communication at Uganda Christian University Mukono, Prof Monica Chibita. “Continue observing the standard operating procedures wear a mask, wash your hands at all times, keep social distancing,” Kaziimba urged. In his later remarks, Kaziimba called upon Ugandans to be more vigilant against the Novel Coronavirus which he said is now even more real than before. 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Earlier during the service, Kaziimba also received a cheque of sh13m (towards the same cause) from the Executive Director of Words of Hope Ministries Uganda, the Rev Canon Captain Titus Baraka.ĭelivering his message to the virtual congregation, Archbishop Kaziimba expressed concern over the increasing cases of theft of Church land.Ĭiting the case of one Rev Mikka Mugavu, the parish-priest of Kisowera Church of Uganda, Mukono, who was recently attacked by people reportedly claiming ownership of Church land, Kaziimba asked government to protect priests and all religious leaders from violent land grabbers. Open Ball Grabbers.zip, next run installer Ball Grabbers.exe 2. She subsequently donated sh20m towards the servicing of the Church House debt. In her latter remarks, Kamya thanked the Archbishop, Samuel Stephen Kaziimba Mugalu, for his visionary leadership, goodwill and prayers. She was delivering her thanksgiving remarks during the virtual Sunday Service at the palace of the Archbishop of the Church of Uganda in Namirembe on Sunday (August 29). Smart user interface-The site has attractive graphics. She said corruption is a general problem , whose eradication requires the cooperation and combined efforts of all Ugandans. Quick process-With no need to create a user account, ig hack lets you crack Instagram accounts in five minutes or less. You will not be able to play multiplayer on bnet or w3champions server, but thats all. Welcome to this week's Software Spotlight video, where we discuss how to use the link grabber Chrome extension.You can find the extension here: https://chrom. KAMPALA - The Inspector General of Government, Hon Beti Olive Namisango Kamya has called for collective efforts in the fight against corruption.īeti Kamya (L) shares a moment with Margaret Kaziimba, the wife of the Archbishop. what do you mean just install the frozen throne client ver 1.30 into a c:warcraft3 directory, on your hard drive, with your old cd key, and refuse the prompt for upgrading. Of the 77 brown-eyed newborns, almost all (73) still had brown eyes at the age of two. Two years later, Ludwig and her colleagues found that of the 40 blue-eyed babies in the study, 11 had brown eyes by the age of two, three had hazel, and two had green. Nearly two-thirds of babies were born with brown eyes, and one-fifth with blue. One study led by Cassie Ludwig, an ophthalmologist at the Byers Eye Institute at Stanford University, tracked 148 babies born at Lucile Packard Children's Hospital in California, recording their iris colour at birth. And sometimes the change appears to happen spontaneously.Įvidence suggests that whether a baby's eye colour changes or not depends a lot on the colour itself. It is a trait that can often leave a lasting impression on us too – eye colour can even influence how trustworthy we find someone.īut, surprisingly, our eye colour doesn't always remain constant throughout our lives – in fact, a wide range of external influences can change it, from injury to infection and sun damage. We might think of our eye colour as one of our defining physical traits, as personal to us as the shape of our nose or how much our ears stick out. By his second birthday, however, the pictures revealed he had become a happy toddler with eyes the same dark brown shade as his father's, with all trace of the dark grey of those early photographs gone. The first pictures of the new-born baby that flashed up on our family chat showed a charming, surprised-looking face with wide, slate-grey eyes – similar in shape to his father's brown eyes, but closer in colour to his mother's green. Dubai Creek Tower May Not Be Recognized as the Tallest Buildingįounded in 1969 in Chicago, Illinois, the Council on Tall Buildings and Urban Habitat (CTBUH) is a non-profit authority on tall buildings. The Chairman of Emaar Properties, however, previously verified that the skyscraper would be 328 ft (100 m) taller than the Burj Khalifa.īut an August 2017 film shown in Dubai Mall indicated that the skyscraper would be between 4,265 and 4,410 ft (1,300 and 1,345 m) in height.Ĭonsidering that we have already seen much taller projects such as the X-Seed 4000 and the Tokyo Tower of Babel, it is possible to construct a structure that reaches a similar height using multiple wires and distributed supports on two sides. Like the Burj Khalifa, the precise height of Dubai Creek Tower will only be revealed during the opening. Either way, it will be the world’s tallest man-made structure, if ever completed. Since it is more of a spire than a building, some estimates put its final height at 4,413 feet (1,345 meters). However, the official final height has not been announced. According to the initial rumors, the Dubai Creek Tower will be a 3,045-foot-tall spire with around 200 floors. The construction of the tower is anticipated to span many years. Nearly 15,000 workers were employed on it at one time. It’s expected that the harbor project’s 2.3 mi 2 (6 km 2) of land will make it the city’s cultural and historical epicenter while being three times bigger than Downtown Dubai, the city’s tourism hub. This neighborhood is located close to Ras Al Khor National Wildlife Sanctuary. The Dubai Creek Tower will serve as the centerpiece of Dubai’s new metropolitan area, Dubai Creek Harbor. Location of the Tower Dubai Creek Harbor and Dubai Creek Tower.Ībout 5 miles (8 km) to the east of the Burj Khalifa is where you’ll find the location of the Dubai Creek Tower. The encircling viewing spaces and gardens planned for the building’s higher levels are meant to evoke the grandeur of another old marvel, the Hanging Gardens of Babylon, which was also built in an arid setting. And it happens that the Burj Khalifa designers were also influenced by the same flower. When designing the Dubai Creek Tower, architect Santiago Calatrava also took inspiration from the lily, a flower that thrives in dry areas. These exterior tension wires and the structural base of the Dubai Creek Tower are planned to be illuminated in a dynamic fashion. Steel wires connected to a concrete shaft create a sleek and elegant framework for this ultra-tall skyscraper. In the end, they appear to be inspired most by the Eiffel and some by the Gardens of Babylon. The tower’s designers looked to Islamic architecture’s classic feature, the minaret, as well as the Hanging Gardens of Babylon and Western landmarks like the Eiffel Tower for inspiration. The city of Dubai’s aesthetics and architectural enhancement have been the primary focus of the Dubai Creek Tower. But will it ever be completed? Dubai Creek Tower’s Design And the tower is expected to cost around $1 billion to build. The Spanish architect Santiago Calatrava conceived the plan for the Dubai Creek Tower. The operator of the Dubai Creek Tower and its environs, Emaar Properties, briefly halted construction in 2020 due to COVID-19. As of the year 2023, construction has only reached the basement level. The Dubai Creek Tower aims to surpass the Burj Khalifa in Dubai, currently the highest structure in the world, by 328 feet (100 m), if not more. The original deadline for completion was 2020, when the World Expo in Dubai would start. In Dubai, a 200-story skyscraper known as the Dubai Creek Tower is being built to a height of at least 3,045 feet (928 meters). The 1946 biopic Till The Clouds Roll By, featuring Robert Walker and based on the life of composer Jerome Kern, was among the first films to use a soundtrack album. Following The Wizard of Oz, she starred in Babes On Broadway, Ziegfeld Girl, all of which were released in 1941, and Meet Me In St Louis (1944). The beginning of World War II had almost no effect on the success of cinema musicals, and Garland continued to be a prominent figure in the field. Judy Garland, like Dorothy, sang one of the greatest movie songs, “ Somewhere Over The Rainbow,” with music by Harold Arlen and lyrics by Yip Harburg (Moritz, 2017). Another 1930s classic is The Wizard of Oz (1939) – one of the most famous musicals of all time. Love Me Tonight, a 1932 classic by Rouben Mamoulian, created a synthesis of music, character, and story so complete, according to Basinger, that it has “never really been surpassed” (Bayard, 2019). MGM’s greatest musicals were supervised by Arthur Freed, who demanded that musical numbers progress the plot. In the 1930s, the RKO cycle of films starring Fred Astaire and Ginger Rogers was the only competition for Warner Bros.’ Berkeley films. Thus Berkley was not the only king of the film musicals in the 1930s. The director is one of the most creative figures of that time, inventing new film patterns, stylizations, and props to enhance the picture of each film musical (Nina Penner, 2017). The usage of motion cameras in Berkeley’s pictures like Forty-Second Street (1933) allowed the audience to feel like they were a part of the choreography (Hodgkinson, 1975). The unique and alluring view shots of dancers were produced by Busby Berkeley, a Los Angeles native who choreographed and directed 19 cinematic musicals in the 1930s. This decade had its distinction – focus on dancing. The cinemas were flooded with mediocrity, which quickly drove the all-talking, all-singing, all-dancing musicals out of business.ĭespite the decrease in musical film quality in the 1930s, it is still marked as a time of few great musicals. At times it seemed to the public as if every film they saw was a musical, and each of them was the same (Hodgkinson, 1975). Indeed the number of musicals produced in 19 dropped dramatically, not only in quantity but also in quality. Many historians argue, however, that the Depression killed off early musicals by 1930, only to resurrect them in 1933 (Moritz, 2017). In addition to posing challenges, this provided an incredible artistic opportunity for those who relocated from the theatre. There was no established winning strategy and no proven methodology for musical filmmaking in comparison to the stage. Metro-Goldwyn-Mayers Studios (MGM) secured the first Academy Award for a musical film for The Broadway Melody in 1929.ĭespite the industry’s rapid growth, film musicals emerged as a new genre that directors were eager to explore. Within a few years, film musicals became popular and loved globally. film starring Al Jolson, was released in 1927, while having only seven songs and a few lines of screen speech, had a significant impact on the cinema business. This is the first feature-length “talkie” The Jazz Singer, a Warner Bros. Bringing these daring innovations to the feature film was a no-brainer and a statement of impudent freshness and vitality. In the 1920s, the interminable lines of high-kicking chorus women that characterize classical music were considered both sexually provocative and strikingly robotic in their clockwork-perfect performances (Griffin, 2018). Long before sound was born, musical roots can be seen in the early film, for which researcher Tom Gunning created the name “cinema of attractions.” The cinema of attractions elicits a high level of awareness of the film image, piquing the audience’s interest, and renouncing narrative cinema’s storytelling role. Step 1: Select a colour for your monster and take a square paper of that colour. Begin by making your bookmark corner- step by step instructions: If the video instructions are not for you, here is how to make this easy paper bookmark following step by step photo instructions Let’s learn how to fold an origami bookmark corner First you will need to make a square out of your paper. RELATED POST: CORNER BOOKMARKS KIDS WILL LOVE How To Make An Origami Monster Bookmark I hope you enjoy this origami monster bookmark tutorial! Supplies Needed For this Bookmark Corner If you’re kids love monsters then we have plenty of monster book ideas and a great monster felt craft tutorial. You could also make a bunch of these bookmarks to give out as party favors or even as a non-candy, allergy-safe alternative for Trick or Treating. Or get creative and use any shapes you like for your bookmark designs! Fold one corner of the triangle back up toward the top point, except now you are going to tuck the top part into. This will create the pocket for your corner bookmark. There are two layers of paper, and you only need to fold the top layer down. You’ll Love These Origami Monster BookmarksĬorner bookmarks are all the rage these days and this bookmark craft uses simple origami folding techniques to create a fun, sturdy page marker for any book.Ĭustomize your monster corner bookmark with any color paper you like and use our free printable template for the eyes and tongue. Fold ONE of the top points down toward the middle of the folded edge. You can draw pictures on the corner bookmark, or. How To Make An Origami Monster Bookmark Easy Sticky Note Origami - BookmarkSimple & Easy Level In this video, you can learn how to make easy origami bookmark out of sticky note paper. The simplest way to use this origami corner bookmark is to fold it, then decorate it with crayons or markers.Unfold the right size, open it, fold the top edge inwards, and close it. Rotate your model 180° and bring both upper edges towards the edge of the triangle, as shown below. |
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